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KMID : 0364820080440020130
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.130 ~ p.134
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Detection of Microbial Growth in an Automated Culture System
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Sung Hye-Ran
Lee Chong-Kil
Jung Yeun-Bok
Song Suk-Gil
Han Sang-Bae
Kim Il Hoi
Kim Jee-Youn
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Abstract
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Modern automated culture systems have increased the isolation rate of microorganisms and shortened the time
to detection, reducing experimental errors in diagnosis of infecting agents. BacT/ALERT 3D system is based on
the colorimetric detection of CO2 produced by the growing microorganisms. In order to evaluate the efficiency
of the detection system, sterility test were performed using 6 bacteria. With standard aerobic and anaerobic bottles
containing the liquid media, both three aerobic bacteria (P. aeruginosa, M. luteus, B. subtilis) and a facultative
bacterium S. aureus were detected up to 1 CFU in 31.44 hr. In addition, growth of anaerobic C.
sporogenes was recognized up to 1 CFU in 15.96 hr. The slowly growing bacteria P. acnes was detected up to
10,000 CFU in 129.36 hr. In comparison with conventional culture method, BacT/ALERT 3D automated culture
system was more sensitive and saved detection time up to 2~10 hr. Therefore, this automated culture system
enables to efficiently detect bacteria in clinical samples and biological medicines.
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KEYWORD
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automated culture system, BacT/ALERT, sterility
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